cell tracker red cmtpx  (MedChemExpress)

Journal: Antibiotics

Article Title: Bacterivorous Ciliate Tetrahymena pyriformis Facilitates vanA Antibiotic Resistance Gene Transfer in Enterococcus faecalis

doi: 10.3390/antibiotics14050448

Figure Lengend Snippet: Images of fluorescence labelled Enterococcus faecalis inside Tetrahymena pyriformis after incubation periods of ( a ) 0.5 h ( b ) 1 h ( c ) 2 h ( d ) 4 h ( e ) 8 h and ( f ) 24 h in Page’s amoeba saline solution—ciliate vesicles with fluorescent E. faecalis are shown in the background ( d – e ). 10 9 CFU/mL E. faecalis was incubated with 10 5 N/mL T. pyriformis at 30 °C for 24 h. Fluorescence was viewed with a green excitation filter set at excitation/emission wavelength of 577/602 nm.

Article Snippet: Preparation of a stock solution of red fluorescence dye, Cell Tracker Red CMTPX (Thermo Fisher Scientific Inc., Waltham, MA, USA), involved warming a dye vial containing 50 μg of red fluorescence dye from its −20 °C storage temperature to room temperature and dissolving the dye in approximately 7 μL of Dimethyl Sulfoxide (DMSO) to achieve a 10 mM concentration.

Techniques: Fluorescence, Incubation, Saline